Unravelling functional neurology: a critical review of clinical research articles on the effect or benefit of the functional neurology approach

Background Functional Neurology (FN), mainly practiced by chiropractors, proposes to have an effect or a benefit on varied clinical cases, from debilitating diseases to performance enhancement in asymptomatic people. Objectives and design A critical review of publications captured in and from the journal Functional Neurology, Rehabilitation, and Ergonomics (FNRE) was performed in order to investigate whether there is evidence on clinical effects or benefits of FN. This review had five research objectives, three relating to the type of literature available through this journal, and two in relation to design and methodological aspects of the included studies. Method All issues of the FNRE journal were searched (October 2017), including a handsearch of their lists of other relevant publications. In order to find evidence in relation to the effect or benefit of FN, the search was restricted to prospective clinical research studies with a control group, claiming or appearing to deal with the topic. The review was undertaken by two independent reviewers using two checklists, one relating to study description, and one on quality. Results were reported narratively. Results Nine articles were found. The FNRE journal contained 168 authored texts, of which 36 were research studies (21%). Four of these were clinical research studies on FN effect or benefit (2%). Another five were obtained through the handsearch. The included studies were conducted on adults or children, symptomatic or not, and investigated various interventions consisting of single or multiple stimuli, of varied nature, all primarily said to be provided to stimulate brain areas. Conditions included attention deficit disorders, attention deficit and hyperactivity disorders, autism-spectrum disorders, cortical visual impairment, traumatic brain injury, and migraine. Balance and the “blind spot” were investigated in healthy subjects. Major design and methodological issues were identified and discussed for all the nine studies; only four were considered as (potentially) appropriate for further scrutiny. However, these were of low methodological quality and, therefore, no robust evidence could be found in relation to the effect or benefit of the tested FN interventions. Conclusions This journal contains no acceptable evidence on the effect or benefit of FN in relation to various conditions and purported indications for intervention.


Background
Functional Neurology (FN), a therapeutic approach founded by a chiropractor, FR Carrick, proposes treatment to alleviate many chronic and even incurable conditions [1]. Given the diversity of symptoms and disorders that "functional neurologists" claim to deal with, ranging from musculoskeletal to neurodegenerative, this approach would have the potential to improve the quality of life of many people.
Therefore, FN interests many practitioners [2,3], including chiropractors, a profession in which this approach may well be spreading. This is mainly achieved through seminars given by private organizations such as the Carrick Institute. The offer of FN seminars is sizable and it is necessary to attend many of them before reaching a certain level of proficiency as a "functional neurologist" [4].
FN is based on the assumption that reversible lesions in the nervous system, especially in the brain, are the cause of a multitude of conditions and that specific clusters of deficient neurons (e.g. neurons on one side of the cerebellum) can be positively affected by various stimuli, including but not restricted to manipulative therapy [1,5].
For example, in a book chapter dedicated to clinical cases, the author describes the FN approach of an attention deficit and hyperactivity disorder (ADHD) patient as well as of a case of low back pain with spinal root compression [6]. In the first case, a FN diagnosis of right cerebral hemisphere and left cerebellum dysfunctions was made. The second case led to a FN diagnosis of right hemisphere dysfunction, meaning that for two such very different types of conditions an almost identical FN diagnosis may be provided. Both cases were treated in the manner of FN with joint manipulations, breathing exercises and nutritional support. The only difference was that, for the case of ADHD, treatment was complemented with sound therapy and spatial rearrangement exercises.
This approach does not appear to be generally accepted in classical neurology, and although many, if not most, of the diagnostic tools used in FN are also known in classical medicine, the interpretation made by "functional neurologists" is probably not always known or understood outside FN, such as their use of the "blind spot" [7], which has evoked questions and criticism [8][9][10][11][12][13].
Further, a recent scoping review on the topic of FN [1] found that despite the extensive list of supposed FN indications, only a few of these appear to have been described in the scientific literature, with an apparent lack of studies in relation to treatment effect, i.e. a lack of studies with robust design. However, this scoping review investigated FN in a context of chiropractic manual therapy and it is therefore possible that evidence might exist on FN interventions that do not include an element of manual therapy (which is not systematically used by "functional neurologists"), for which reason this area merits further investigation.
Many chiropractors are using, at least, elements of FN on their patients, but as the chiropractic profession in many countries is legally recognized and hence expected to be evidence-seeking and evidence-accepting, it is important to investigate the level of such evidence regarding FN, including its clinical effect or benefit. Such evidence is required for FN to be regarded as credible. Obviously, this requirement would be the same for any therapeutic approach when, as in this case, it is not an accepted part of mainstream medicine, and in particular if the theories on which they rest are not generally considered to be plausible in the light of present-day state-of-the-art knowledge.
Unfortunately, scientific literature that covers FN topics has already been found by the present authors to be difficult to capture, making a systematic search difficult when searching for indexed literature in scientific databases. One of the most well-known authors within FN previously directed us to the journal Functional Neurology, Rehabilitation, and Ergonomics (FNRE) (G Leisman, personal communication). This journal is published by the Nova Science Publishers group [14], not indexed in Medline or Scopus and therefore somewhat difficult to find. It is affiliated to the International Association of Functional Neurology and Rehabilitation, an FN organization promoting this approach, including through research activities such as the FNRE journal.
Although this journal states under its "aim and scope" to deal with topics other than FN, a list of neurological disorders is provided with diseases and traumatisms of the brain listed first under its "description of the field covered". Therefore, this appears to be a major source of information on the FN approach. The FNRE journal was previously searched for its scientific contents in relation to FN in a chiropractic context but resulted in only three relevant articles [1]. Nevertheless, relevant information on FN in general might still be found in this journal, as manipulation is only one of the therapeutic tools available to "functional neurologists".
We therefore decided to perform a critical review of all publications in this journal to investigate the evidence for clinical effect or benefit of FN. Specifically, our research objectives were: 1-To define the proportion of articles that are research studies (i.e. not narrative reviews, discussion papers, poster abstracts, abstracts, editorial material, or public relation information) in the FNRE journal.
2-To define how many of those are clinical research studies that purported or appeared to deal with effect or benefit of FN.
3-To describe which indications and FN interventions were studied in the clinical research studies captured through the FNRE journal.
4-In these studies, to establish whether the design and overall study method were suitable for research into the effect or benefit of FN. 5-To describe the evidence available in relation to the clinical effect or benefit of FN, taking into account some minimal methodological criteria.

Search strategy for information and screening procedure
All issues of the journal Functional Neurology, Rehabilitation, and Ergonomics were obtained in October 2017. At this period, all of its six volumes and twenty-four issues, edited between 2011 and 2016, were searched for research studies on FN effect or benefit, based on titles and abstracts, and, when needed, on full texts. No issues were published before 2011 or after 2016, at the time of writing this report (December 2017 to January 2018).
The texts in these issues were blindly screened by the authors, according to predetermined definitions of which articles would be considered acceptable, i.e. considered as research studies (defined in the section Inclusion and exclusion process of articles). Thereafter, the two authors extracted from all the research articles those articles that were clinical research articles reporting on effect or benefit of FN.
Most issues of the journal FNRE contain a section entitled "IAFNR News and Events", where various types of information about the International Association of Functional Neurology and Rehabilitation and its members is reported. Within this information, lists of recent publications in peer-reviewed journals authored by members of the International Association of Functional Neurology and Rehabilitation were available. These reference lists were independently searched by the authors to find additional clinical research studies in relation to effect or benefit of FN. Only titles of published or scientific articles accepted for publication were considered in these lists, which mixed published, accepted, and submitted articles, as well as conference papers.

Inclusion and exclusion process of articles
Step 1: In order to define the proportion of articles that were research studies in the six volumes and twenty-four issues of the journal FNRE, the total of texts was counted twice by ALM on the basis of the table of contents of each issue. We defined as a text a written script introduced by a title for which at least one author's name was mentioned. Therefore, texts may include written scripts as varied as scientific articles, abstracts, editorials, and letters to editor.
Articles were considered as research studies when they had 1) one or several research questions or research objectives and 2) a methods section that explained the process of data collection and data analysis. This means that discussion papers, narrative reviews but also case reports would not be counted as such. We did not include research information presented solely in abstracted form, such as posters and conference proceedings, as they do not contain full information of the study project. At this stage, we did not differentiate experimental studies from clinical studies and we also included studies that dealt with other topics than FN.
Step 2: Research studies were included as clinical research studies dealing with effect or benefit of FN if 1) the intention to investigate an effect or benefit was obvious, searching throughout the articles for words such as "effect(s)", "effectiveness", "improvement(s)", "improve", "recover", "recovery", or "benefit(s)", and 2) the intervention that was investigated had the hallmark of FN, as it was described in a previous review [1]. Furthermore, research studies were considered as clinical when the investigated intervention was clearly known or identified as already used in clinical practice, specifically within FN.
Any intervention that included a stimulus said to be directed to the nervous system could be included, given that the FN approach had been previously described as consisting of almost any kind of stimulus purported to stimulate the nervous system, especially the brain [1]. This could also be multifaceted, i.e. consisting of various stimuli, being or not complemented by nutritional counseling or supplements.
These studies could report results on symptomatic or asymptomatic subjects and, if subjects were symptomatic, symptoms could be of any kind, according to the wide supposed scope of FN.
Hence, at this step, any full text clinical research studies regarding FN effect or benefit could be included, regardless their study design, i.e. appropriate or not to investigate an intervention effect or benefit but case reports, narrative reviews and discussion papers were not included for the reasons explained above. Also, articles were included regardless of the type of subjects and FN intervention investigated.
Step 3: For further scrutiny, we searched for prospective studies with at least one control group.
We selected those studies that related to the effect of an intervention, if it was investigated in one of two ways: the intervention could be compared 1) to a sham procedure (to control for the placebo effect), or 2) to an intervention already known to be effective, i.e. already tested against placebo.
Also prospective studies with other types of control groups (e.g. control group subjected to an intervention accepted in medical practice for the investigated condition) were included in order to investigate benefit of intervention. Studies could be included whether they were conducted or not with a random allocation.
Retrospective studies and studies without one or several control groups were not considered suitable at this step.

Extraction of information
Two checklists were created for the review: one related to the description of the studies (Table 1) and one to their methodological quality ( Table 2). The latter consisted of two parts. The first part concerned all the clinical research studies included and contained only one item in relation to the design of the study and its potential appropriateness to investigate an effect or benefit.
If the design was not considered appropriate, from a methodological perspective, remedial propositions were given in order to promote the conduct of studies that would be able, in term of study design and methodology, to investigate whether the FN approach has an effect or benefit.
If the study design was considered potentially appropriate to study effect or benefit of an intervention, the article was reviewed for further quality assessment, based mainly on some items proposed in the Cochrane recommendations [15]. For this we developed a seven-component quality checklist consisting of five risk-of-bias items, one item relating to external validity and one to unsystematic methodological errors as described in Appendix. Sometimes, other glaring methodological problems were mentioned in the text.
These items were added up for each article and percentages calculated taking into account the possibility of the occasional item being irrelevant (not applicable). No cut point was set for acceptability but the final score was instead used to illustrate, in a very basic way, the level of scientific rigor and credibility of the included articles.
All selected articles were reviewed independently and blindly by the two authors. Information was sought throughout the text but not in the abstract and discussion sections. Data collected in the two checklists by the two authors were compared and discrepancies resolved by consensus.
Initially, a third checklist, related to the results of the effect or benefit studies with the most robust designs was considered but finally not needed, as will be evident further in this report.

Data synthesis
The results of the selection process ( Fig. 1) served to provide information for our two first research objectives. Tables 1 and 2 were created for the remaining research objectives. In both tables, articles were presented consecutively by year of publication. On their basis, a narrative synthesis of the collected data was provided for each research objective.

Results
Proportion of research studies and clinical research studies dealing with effect or benefit of Functional Neurology (research objectives 1 and 2) As illustrated in Fig. 1, in a total of 168 texts found in the journal Functional Neurology, Rehabilitation, and Ergonomics, 36 were identified as research studies (clinical and experimental). Among them, four were identified as clinical research studies dealing with effect or benefit of FN [16][17][18][19]. This means that 21% of the texts contained in its six volumes, published from 2011 to 2016 are research studies in general, and 2% of all texts are clinical research studies on the effect or benefit of the FN approach.
One of the research articles (Castellanos et al. [24]) was defined as not dealing with FN effect or benefit but was nevertheless reported on because it dealt with other relevant clinical issues. This scientific article was counted as a research study but not as a clinical research study, which would not change substantially the percentage of the latter. Nevertheless, for the sake of interest, it  will be described with the other studies, resulting in five relevant articles. The handsearch in the section "IAFNR News and Events" of the FNRE issues provided four additional clinical research studies in our area of interest [20][21][22][23], all from other scientific journals. A total of eight clinical research studies on FN effect or benefit were therefore included in the review in order to fulfill our three other research objectives, plus the additional clinically relevant study [24], bringing the number to nine.
Study objectives were not always clearly stated, and if (as was sometimes the case) the introduction was also unstructured and confusing, the whole text had to be scrutinized to identify the purposes of the studies. For this, we searched for terms such as "effect" and "effectiveness" in the texts. As shown in Table 3, the intention No mention about any potential conflict of interest ADHD Attention deficit and hyperactivity disorders, ADD Attention deficit disorders, ASD Autism-spectrum disorders to investigate an effect or some type of benefit of FN was clearly stated by the authors in eight of the included studies. The ninth (Castellanos et al. [24]) appeared to us first as having the same intention (as shown in Table 3), however, after further scrutiny and discussions, it was not considered to intend to investigate treatment effect or benefit of FN. None of the authors reported explicitly having an ethics approval from an identified ethics committee with an identification number of the application and approval ( Table 1, col.7). Nonetheless, one reported to have ethics approval, two reported to have an ethics approval from an unidentified review board, which may or may not be the same as an official human research ethics committee, and another reported to be "approved" without more information. The remainder (n = 5) either mentioned that they acted in accordance with some ethical recommendations or provided no information.
As for conflict of interest, this was not mentioned at all in six of the reports, whereas two declared to have such a conflict and one declared to have none. Nevertheless, in the latter and in two of the "undeclared" articles, we identified potential conflicts of interest and, in the two "declared", we identified some additional potential conflicts of interest (Table 1, col.8).
The choice of study design to investigate effect or benefit was often not respected and, in those studies where a full methodological assessment was done, the quality scores were low, never reaching 50%.

B. Description in relation to indications and
Functional Neurology interventions studied (Research objective 3)

Indications studied
The selected studies included either symptomatic (n = 6) or asymptomatic (n = 3) subjects: adults (n = 5) and children (n = 4) ( Three studies reported on subjects diagnosed with ADHD (Table 1, col.2). One of them included also subjects diagnosed with attention deficit disorders and another reported on subjects diagnosed with ADHD or with autism-spectrum disorders. These two studies mixed these types of subjects on the basis that they were supposedly identified as having one brain hemisphere deficient compared to the other, referring to the FN concept of hemisphericity [25]. Two studies had balance as its main topic, which was investigated on healthy subjects without balance or gait disorders ( Table 1, col.2 & 3). The last four studies reported on: i) cortical visual impairment, ii) brain function asymmetry in healthy subjects, iii) traumatic brain injury in, apparently, post-stroke subjects, and iv) migraine in people having a migraine episode (Table 1,

Interventions studied
While the intervention was well described by most of the authors, in two articles this was poorly reported, making it difficult to fully understand what the FN approach contained [22,24]. The FN interventions consisted of a single modality (n = 5) or were multifaceted and individualized (n = 4) ( Table 1, col.4). Manipulative therapy (n = 1), motor sequencing training (n = 1), whole body rotation (n = 2), and pneumatic ear insufflation (n = 1) were tested as single modalities of intervention. Multifaceted programs were of different kinds, consisting of a combination of visual stimuli (n = 1), of a neuropsychological rehabilitation program, complemented or not by one or several physical modalities (n = 1), or of a combination of mainly motor, sensory and cognitive stimuli (n = 2).
The total numbers of treatment sessions and their frequency were disparate, ranging from a single intervention of 40 s to 4-15 months of a home program, which probably involved daily stimuli (Table 1, col.4). Outcome measures used to assess effect or benefit also varied from one study to another but all assessed them at least before and after intervention, at various time points (Table 1, col.5 & 6).

Consideration of major design and methodological issues (research objective 4)
All studies included in this review had major design and/or methodological problems in relation to study the effect or benefit of FN. In this section, the main issues that we identified are reported.
A. Study designs unable to detect effect or benefit of interventions

Studies without control group
Two articles reported on outcome studies (Carrick et al. [16], Bousquet [19]) and one on an outcome study or on a case series (it was unclear if this was a prospective or a retrospective study) (Leisman et al. [23]). Unlike reported by the authors of this study, we did not consider it as a pilot study. The reason is that a pilot study may be used for several reasons before conducting a clinical trial but in such study it is not suitable to test clinical hypotheses and provide estimates of effect [26], which was the case in the article of Leisman et al. [23]. These three articles did not include a control group and were therefore not included in our final analysis.

Study with control group that was not concomitant
Malkowicz et al. [20] had as their research objective to study "the effects of an intensive visual stimulation treatment program on visual recovery" in children diagnosed with cortical visual impairment. This study consisted of a retrospective analysis of a clinical database with an external control group from a previously published study and was not included in our final analysis. The reasons for this were that this study lacks two important aspects: (i) study subjects were not included in the study at about the same time, meaning that the disorder and treatment may have become different over time, and (ii) as the intervention-and control-groups were not included under the same circumstances, it is likely that they were not comparable on all or most variables, apart from the tested intervention. This makes it difficult to ensure that it is the treatment that matters and not some other circumstances. Nevertheless, the design of this study would be suitable to provide preliminary insights into a rare condition with poor prognosis. However, in the present study, since the authors state that "time is the factor of essence", it would have been important, when  comparing results in two different groups, that the visually impaired children were all at the same stage of the disease and assessed at similar time intervals, which is not clear in this report. The results may well be encouraging, for which reason this study could be considered as a first step to inspire a proper randomized controlled trial.

Study examining mechanisms of intervention rather than effect or benefit
An additional study, Castellanos et al. [24], is worth mentioning. As previously stated, we first assumed that it studied effect or benefit but, on closer scrutiny, it became clear that it did not claim directly this but that it dealt with other relevant clinical issues, namely the question whether the neurophysiological measurements of brain activity ("entropy" and "complexity") before and after treatment were linked to the clinical state of the subjects. To study this, the authors used a healthy control group for comparing their baseline values to the baseline values of the cases, to see if brain "entropy" and "complexity" were different in the two groups. After a neuropsychological rehabilitation program, a comparison was made again with the previously obtained baseline values of the healthy untreated group to see if the study subjects now resembled more the healthy control group than they did at baseline. The results were measured against information on activity of daily living. In other words, our interpretation was that the authors tested if the brain function issues that they addressed through intervention had a clinical value.

B. Studies potentially able to detect effect or benefit of Functional Neurology
Daubeny et al. [21] ( Table 2, row 3): The best study, in terms of methodological quality, still had a quality score of only 4/9 (44%). In this randomized controlled trial with a sham treatment, the "blind spot" was measured before and after joint manipulation and found to change in a particular pattern. However, neither reliability nor reproducibility of the measurement of the "blind spot" were tested within the article or reported as reproducible  [20] Intern J Neuroscience -Introduction/Objective "…the authors were particularly interested in studying the effects of an intensive visual stimulation treatment program on visual recovery." (p.1018) -Discussion "…it can be seen that visual stimulation programs improve a brain-injured child's ability to see significantly more than that of an individual not receiving visual stimulation." (p.1032) Daubeny 2010 [21] Int J Disabil Hum Dev -Title "Effects of contralateral extremity manipulation on brain function" -Discussion "The Sham manipulation did not have such an effect supporting that observations that it is the manipulation itself that is causing the changes in brain function." (p. not available) Leisman 2010a [22] Int J Disabil Hum Dev -Title "Effects of motor sequence training on attentional performance in ADHD children" -Abstract "Rhythm feedback training appears to have a significant effect on clinically observed changes in behavior in attention-deficit/hyperactivity disorder…" (p.275) Leisman 2010b [23] Int J Adolesc Med Health -Title "The effect of hemisphere specific remediation strategies on the academic performance outcome of children with ADD/ ADHD" -Discussion "We here attempted a pilot study to determine if treatment that is preferentially aimed at a hypothesized interactive right hemisphere in ADD/ADHD children would have an effect on their sensory motor performance, as well as on cognitive function related to attention focus." (p.281) Carrick 2011 [16] Funct Neurol Rehabil Ergon -Title "The effects of whole body rotations in the pitch and yaw planes on postural stability" -Results "To investigate the effects of the Rotation, one tailed t-test for paired observations with…" (p.174) Castellanos * 2012 [24] Funct Neurol Rehabil Ergon -Title "Restoring the brain entropy and complexity after rehabilitation of traumatic brain injury" -Discussion "After rehabilitation, the local networks recover, understanding recovery as an approach to control values of organization." (p.212) Carrick 2013 [17] Funct Neurol Rehabil Ergon -Title "The effect of off vertical axis and multiplanar vestibular rotational stimulation on balance stability and limits of stability" -Method "To evaluate the effects over time the rotational stimulation could have on the balance of the subjects, each…" (p.347) Sullivan 2013 [18] Funct Neurol Rehabil Ergon -Introduction/Objective "Our task was to investigate the effectiveness of this simple, non-invasive, low-cost and readily available bedside therapy." (p.94) -Discussion "…, it would seem unlikely that the observed effects are due particularly to stimulation of…" (p.102) Bousquet 2015 [19] Funct Neurol Rehabil Ergon -Title "The perceived effects of hemisphere integration therapy on students with identified right hemisphere weakness" -Conclusion "Before this study, there was no research regarding the effects of HIT on students with ASD." (p.292) *This article was later considered not to deal with effect/benefit or reliable on the basis of other studies. For this reason, it is not known if the findings in the present study can be trusted or if the findings could be fluctuating in a meaningless manner. Another problem is that the authors failed to describe clearly that its study subjects did not have a special interest or preconceived ideas in relation to manipulative therapy and the "blind spot", as the origin of the study sample was unreported. This is important if study subjects could have been able to, willfully, change their visual reporting during the experiment. Further, as the validity of the "blind spot" as a neurological test with the ability to change with manipulative therapy has been questioned [8,11,13,27], blinding in all possible ways is particularly important, i.e. also of the statistician, which was not described in the report. Therefore, although this study is a randomized controlled trial, it presents major methodological issues that potentially affect the validity of the reported results.
Leisman & Melillo [22] ( Table 2, row 4): This study, 2/ 8 (25%), failed to report the use of a blind assessor and, therefore, it is not clear if the outcome could have been positively influenced or further aggravated by the absence of information on reproducibility of the collected data. Also, it was not reported if all study participants stayed to the end or even if they were all included in the final analysis. Although this study, apparently, reported on two randomized controlled trials consisting of one group of children with ADHD receiving or not receiving an intervention and a second group of "normal" age-matched children also receiving or not receiving (the same) intervention, we were unable to interpret the results. In fact, the authors did not clearly explain the results that were cryptically presented in a table and a figure and it was not clear to us exactly how comparisons were made between the four groups. Other methodological quality issues appeared when we completed our quality checklist.
Carrick et al. [17] ( Table 2, row 8): Another report, 2/ 8 (25%), consists of two studies, containing analyses taking into account posturographic reactions in asymptomatic subjects, who were subjected to whole-body rotations in different planes. Eight and six outcome variables respectively were tested before and after the interventions at three different time intervals but there was not a control group that received no intervention. Admittedly, it would be difficult to make comparison to a sham whole-body intervention. For this reason, it would have been suitable to compare intervention to some other type of control in order to see if the tested intervention had some benefit. It would, also, have been possible to compare "correct" to "incorrect" intervention, to see if study subjects reacted differently to these in a logical manner.
In fact, when scrutinizing the research design, it gave the impression that this was the purpose, i.e. to compare "correct" to "incorrect" intervention. The study subjects were originally classified in relation to their different postural types in relation to "pitch" and "yaw", i.e. the preferred position related to the head position when standing on a "perturbing foam cushion" with their eyes closed and the head rotated ("yaw") and the head extended or flexed ("pitch"). Study subjects were divided into four groups according to their "pitch" and "yaw" predominance, after the foam cushion test, i.e. head in flexion, head in extension, head rotated to the left, head rotated to the right, and the various combinations of these.
Intervention was provided to all these four subgroups but not in the same way. The intervention was stated to be different in relation to the directions of "pitch" and of "yaw". Thus, the study subjects were randomly allocated to receive a treatment (i) in the same directions as their preferred "pitch/yaw" postural reaction at baseline; (ii) in the same direction as their "pitch" position but opposite to their "yaw" position; (iii) in the opposite direction as their "pitch" position but in the same as their "yaw" position; or (iv) in totally opposite directions.
Presumably, although we did not understand the explanations of why this was done and what was expected, this design could be used to analyze if study subjects in the different groups would react differently on intervention, according to whether the classification and the intervention (i) matched, (ii) and (iii) matched partially, or (iv) did not match (as explained above). This sort of analysis would (perhaps) be able to provide information on whether the rationale for the intervention was correct or not. In addition to our checklist items, we noted that there were far too few study subjects in these two studies for the large number of tests and far too few values in each cell to allow for meaningful statistical analysis in the two reported studies. Also there was no report on the reproducibility of the "pitch" and "yaw" findings, meaning that the before-after measurements could be fluctuating regardless of intervention.
Further, the authors provide a very detailed and, in our opinion, confusing results section. This makes it difficult to understand if they actually tested whether the various intervention strategies based on the "pitch/yaw" classification resulted in different results for their many outcome variables. Only in the second study (p.355 l.15) is one of the stimulation groups mentioned. We were therefore confused as to whether the authors ignored the results of the matched intervention and only studied the change over time for different types of interventions and for different types of "pitch/yaw" classifications. This approach would in fact correspond to the study design of an outcome study. In other words, it looked to us as if they simply compared the baseline measurements at the three follow-up time points (i) for all treated subjects according to their base-line classification (p.346 under "Research Questions, l.4") regardless if their treatment was matched to their classification or not, and (ii) for all four combinations of body rotation stimulation (p.346 under "Research Questions, l.5") regardless if the classification group was treated in a "matched" manner or not. If we assume this to be the case, this confusing and complicated report would not have used its clever design to its full potential. This study also fails in other methodological aspects, as reported in Table 2.
Sullivan [18] ( Table 2, row 9): This author reported on a prospective case series, where the clinician was also the assessor of the treatment outcomes, which reached a quality score of 2/8 (25%). This study seems to reflect the work of a clinician who has tested an original neurophysiological theory in his clinical practice. Some but not all study subjects (3/13) were subjected to a sham treatment but without random allocation, hence lacking a proper control group. Nevertheless, the results seem encouraging and might incite a future proper randomized control trial, as suggested by the author. Therefore, this study could be considered an interesting preliminary study, to see if the topic is worthwhile being pursued, but does not allow the author to deal with any effect or benefit of the tested intervention, i.e. pneumatic ear insufflation in the treatment of migraine.
Description of the evidence available in relation to clinical effect or benefit of Functional Neurology (research objective 5) Out of the nine studies that potentially dealt with effect or benefit of FN interventions, four were considered to, at least somewhat, be able to produce such answers. The quality scores of our quality checklist ranged from 25 to 44%, indicating an overall substantial risk of bias mainly in relation to: 1) blinding of study subjects, assessors and statisticians, and 2) concealment of random allocation. In addition, the outcome variables were never stated to be reliable or reproducible, making it reasonable to suspect that the reported results could be attributed to their inherent variability.
In light of these methodological short comings, we did not consider the results of these studies dependable. Therefore, we found no acceptable evidence that could support the notion that the FN approach has an effect or a benefit on the supposed indications tested, whether this was done on symptomatic or asymptomatic subjects. For this reason, the results of the various studies were not reported or illustrated as initially planned.

Discussion
Out of a total of 168 texts published from 2011 to 2016 in the FNRE journal, 36 were identified as research studies in general, but only four could be classified as clinical research studies potentially investigating FN effect or benefit. A total of nine articles, five from the FNRE journal plus four from three other scientific journals (identified through the journal FNRE), were included for further description and analysis.
Due to design and methodological issues, no acceptable scientific evidence was found in relation to effect or benefit of various FN interventions. This was the case for studies on symptomatic children, mainly suffering from neurodevelopmental disorders, for symptomatic adults, suffering from migraines or traumatic brain injury, and also for asymptomatic adults on whom balance or "blind spot" changes after FN interventions were investigated. All had the hallmark of FN, i.e. targeting different parts of the brain, but did not bring any evidence on indications for treatment or for the best match between condition and intervention.

Considerations regarding the type of literature captured
The few research studies in general, i.e. covering FN topics or not, indicates that the authors who publish in this journal are more inclined to write discussion papers or narrative reviews than research studies. Further, within this small group of research articles, only a few were relevant for our review. Nevertheless, given the small percentage of research studies, it could be argued that research on FN has or has not been published extensively outside this journal. But, in a previous review, the present authors also noticed that only few scientific articles were easily available in general on the clinical aspects of FN but from a chiropractic perspective [1]. Thus, the present critical review also reveals this paucity of research evidence in relation to the effect or benefit of FN.
Obviously, academic inclined clinicians have a need to read and exchange. The FNRE journal seems to provide ample opportunities for this but there is an obligation on all scientific journals not only to discuss and claim but also to establish the basic scientific facts. This critical review failed to find robust evidence of the latter.

Methodological considerations of reviewed studies
The study design Several studies did not even include a control group and only one compared the intervention group with a sham intervention in a randomized controlled trial.
In studies without control groups, only outcome can be reported; thus it is not appropriate to talk about effect or benefit. The reasons for this are that studies without a control group, showing improvement after intervention, may indicate a true effect, a regression towards the mean, or simply the natural course of a disease that gets better on its own or has its ups and downs, but it is not known what. Thus, the scientific interpretation is usually only of a positive outcome, a potential benefit but certainly not of effect of intervention. This is the main reason why clinicians who see their patients improve cannot claim to have an "effect", only hope there is, as there are no placebo groups possible in clinical practice.
Clearly, most of the authors of the articles included in the present review, as has been found before in another chiropractic research field [28], seem not to be completely familiar with the requirement to be meticulous about matching the research question to the correct research design.

The research methods
Each research design (e.g. surveys, clinical trials, population-based studies) has its specific requirements. These are based on logical rules that are well accepted in the scientific community, although errors and omissions are often observed in the research literature. The articles reported in this review were often non-observant of these rules, at least for those pertaining specifically to randomized controlled trials.
For randomized controlled trials, whether comparing treatment to a placebo procedure or another treatment, well established quality checklists exist, such as that used by the Cochrane collaboration [29]. We did not use this but extracted only few important points, as it was evident that the reviewed literature was deficient and that it would be meaningless to go into details. We conclude that the few items we selected were sufficient to point the reader in the right direction.
In sum, the major finding of this review was the lack of conventional use of research design and method in order to investigate any effect or benefit of the FN approach. Of serious concern was the lack of information regarding approval from an identifiable human research ethics committee. We also noted that although some did report conflicts of interest, some did not mention this aspect or seemed to do so incompletely.

Methodogical considerations of own review
Searching one single journal had the advantage that we were unlikely to miss studies of interest. However, this does not mean that all the scientific literature on the FN field has been covered in the present review. Nevertheless, this was not our intent. The motivation to restrict the search to the FNRE journal was already evoked in the Introduction of this article. In fact, we have previously established that the FN literature was difficult to find [1]. The main reasons for this are that publications on this topic usually are not associated with the key word "functional neurology" and FN proposes so many treatment or intervention approaches (types of stimuli) for so many conditions that it would probably be impossible to design a relevant search equation to capture all the FN literature.
The quality checklist used in the review was specifically designed for our purpose but, potentially, other researchers might select other items to assess the methodological quality (including risk of bias) of the included studies. Nevertheless, given the problems relating to design and methodological issues discussed above, it is very unlikely this would affect the conclusion of the present review.
Also, we adopted a lenient approach for inclusion of the studies in our final analysis, selecting randomized and non-randomized studies, with or without proper control groups. A more stringent selection would have brought even less studies to discuss. This flexibility gave us the opportunity to address design and methodological issues in order to bring the reader, especially clinicians and health care students, some basic knowledge needed to effectively consume research reports. Not all health practitioners have adequate skills and experience in the reading of the research literature. This is despite the need to critically appraise the literature encountered during their career, even when such literature is promoted and produced by their colleagues. This is also true for FN, a movement within which research has a clinical and commercial component [30].

Conclusion
We can conclude that the FNRE journal, with a special interest in FN, contains only few clinical research articles in this field. Further, it is clear that over five years and twenty-four issues of this journal, no methodologically sound studies on the effect or benefit of the FN approach were published. In order to find out if there is, in fact, other relevant documentation on the effect or benefit of FN, a critical review of the scientific publications of its founder, FR Carrick, apparently actively involved in research, may be able to fill in the gaps regarding the scientific state of FN.

Appendix
Items selected for the second part of the quality checklist ( Table 2) and their rationale In relation to study subjects: -Were study subjects stated to be unbiased (blind and naïve)? The reason why it is important that subjects are blind to the nature of the experiment is that subjects may be influenced by their expectations to treatment outcomes. For the same reason and when this is not possible to blind the subjects, i.e. when the intervention is not compared to a sham intervention but to another intervention, subjects have to be at least naïve to the intervention they receive.
-Was allocation to study groups stated to be randomized and concealed? The random allocation and its concealment minimize the risk of selection bias.
In relation to the experiment: -Was the intervention well described? A sufficient description of the investigated intervention is one key element that allows to replicate the study, especially when this is a new and/or multifaceted intervention.
-Was the outcome measure reported to be reproducible or reliable? An acceptable reproducibility and reliability are needed to ensure that the study results are not simply due to normal variations of the measures over time or due to intra and/or inter-examination variabilities when performing the measures.
In relation to the assessment: -Was the assessor stated to be blind to group allocation? When not blinded, the assessor may be influenced by his/her wish to obtain better results in the intervention group compared to the sham/control group.
In relation to analysis and data reporting: -Was the statistician stated to be blind to group allocation? This is for the same reason that the assessor has to be blind.
-Were losses and exclusions reported or obvious in results, tables or graphs? Reporting losses and exclusions, if any, allows to appreciate in which extent this could affect the reported results.